How Can I Extract Pure Nicotine From Tobacco Leaves?

I will use the pure nicotine for our research. We are trying to discover if nicotine has an inhibitory effect against certain bacteria.

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One Response to “How Can I Extract Pure Nicotine From Tobacco Leaves?”

  1. Christophe G Says:

    nicotine makes a nice complex with tartric acid. You can put crushed tobacco leaves in a solution of sodium hydroxide and do a steam distillation. Take the distillate and mixed it with a solution of tartric acid and the precipitate should be nicotine tartrate. You can acidify the tartrate and distill/extract the nicotine out.
    However, the simplest way is to buy commercial nicotine (which will contain some yellowish nicotine oxide) and purify the commercial nicotine with silica gel chromatography.

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